43,450-feature dna microarray Search Results


43,450 feature dna microarray  (Oxford Gene Technology)
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Oxford Gene Technology 43,450 feature dna microarray
(A) Distribution of CbpA across the E. coli chromosome. i) Genome-wide view of CbpA binding in starved MC108 cells. The figure shows ChIP-chip data for CbpA binding plotted against features of the E. coli genome in the form of a genome atlas. The data have been averaged across a 100,000 base pair window. The four chromosomal macrodomains (MD) are labelled ii) CbpA ChIP-chip data for a small section of the E. coli chromosome. The data have been averaged across a 10,000 base pair window. (B) Relationship between CbpA binding and <t>DNA</t> GC content. The graph shows the average CbpA binding signal plotted against the GC content of probes on the DNA <t>microarray.</t> The average GC content of the E. coli K-12 chromosome is shown by a dashed line. Very GC rich and GC poor probes were excluded during microarray design and are thus absent. (C) Effect of CbpA on DNA supercoiling in vivo . Panel i) shows an image of a 1% (v/v) agarose gel containing 2.5 µg/ml chloroquine. Plasmids were isolated from different genetic backgrounds and different stages of growth as indicated. Panel ii) also shows an image of a 1% (v/v) agarose gel containing 2.5 µg/ml chloroquine. Plasmids were isolated from rapidly dividing cells.
43,450 Feature Dna Microarray, supplied by Oxford Gene Technology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) Distribution of CbpA across the E. coli chromosome. i) Genome-wide view of CbpA binding in starved MC108 cells. The figure shows ChIP-chip data for CbpA binding plotted against features of the E. coli genome in the form of a genome atlas. The data have been averaged across a 100,000 base pair window. The four chromosomal macrodomains (MD) are labelled ii) CbpA ChIP-chip data for a small section of the E. coli chromosome. The data have been averaged across a 10,000 base pair window. (B) Relationship between CbpA binding and DNA GC content. The graph shows the average CbpA binding signal plotted against the GC content of probes on the DNA microarray. The average GC content of the E. coli K-12 chromosome is shown by a dashed line. Very GC rich and GC poor probes were excluded during microarray design and are thus absent. (C) Effect of CbpA on DNA supercoiling in vivo . Panel i) shows an image of a 1% (v/v) agarose gel containing 2.5 µg/ml chloroquine. Plasmids were isolated from different genetic backgrounds and different stages of growth as indicated. Panel ii) also shows an image of a 1% (v/v) agarose gel containing 2.5 µg/ml chloroquine. Plasmids were isolated from rapidly dividing cells.

Journal: PLoS Genetics

Article Title: E. coli Fis Protein Insulates the cbpA Gene from Uncontrolled Transcription

doi: 10.1371/journal.pgen.1003152

Figure Lengend Snippet: (A) Distribution of CbpA across the E. coli chromosome. i) Genome-wide view of CbpA binding in starved MC108 cells. The figure shows ChIP-chip data for CbpA binding plotted against features of the E. coli genome in the form of a genome atlas. The data have been averaged across a 100,000 base pair window. The four chromosomal macrodomains (MD) are labelled ii) CbpA ChIP-chip data for a small section of the E. coli chromosome. The data have been averaged across a 10,000 base pair window. (B) Relationship between CbpA binding and DNA GC content. The graph shows the average CbpA binding signal plotted against the GC content of probes on the DNA microarray. The average GC content of the E. coli K-12 chromosome is shown by a dashed line. Very GC rich and GC poor probes were excluded during microarray design and are thus absent. (C) Effect of CbpA on DNA supercoiling in vivo . Panel i) shows an image of a 1% (v/v) agarose gel containing 2.5 µg/ml chloroquine. Plasmids were isolated from different genetic backgrounds and different stages of growth as indicated. Panel ii) also shows an image of a 1% (v/v) agarose gel containing 2.5 µg/ml chloroquine. Plasmids were isolated from rapidly dividing cells.

Article Snippet: The “plus and minus antibody” DNA samples were then labelled with Cy5 and Cy3 respectively before being mixed and hybridised to a 43,450 feature DNA microarray (Oxford Gene Technology).

Techniques: Genome Wide, Binding Assay, ChIP-chip, Microarray, In Vivo, Agarose Gel Electrophoresis, Isolation